When I visited Newburgh, Indiana, at the invitation of Drs. Mythili and Sudheer Gurrams, I made two presentations at the Tri-State Hindu Temple.  First one on the ‘Hindu Wave. Will it survive? Is there a future for Hinduism?” The second one was about the science behind the traditions and customs we observe in Hindu Temples. We observe people applying a tilak, performing Pradakshana, witnessing Abhishekam, squatting in front of Ganesh, sipping a few drops of Teertham, listening to the chanting of Sanskrit mantras, performing Puja to the presiding deities, visiting multiple shrines, and following many other customs. Many of us keep wondering why we do all these things. Is there a meaning behind these observances, and can we explain these customs scientifically? Please read about the science behind applying Tilak on the forehead.
Kumkum/Bindi / Tilak is applied in the center of the forehead or on the sixth chakra, which is located between the two eyebrows. This spot is considered the Ajneya Chakra, the Commanding center. It is believed to be the converging point of major nerves. It is the junction where the highest number of neurons meet, hence described as the most important yet sensitive area. There is spiritual and scientific significance to the concept of Kumkum/Bindu /Tilak.    
The Third Eye Chakra is the center of intuition, spiritual insight, psychic abilities, and higher consciousness. It is associated with intuition, inner wisdom, clarity of thought, and spiritual insight. When balanced, it allows you to see beyond the obvious, trust your instinct, and experience a deeper connection with yourself and the world around you.
This Center controls the entire body. This is the only Chakra exposed to the outside, while the rest are covered by either clothes or hair. This is the chakra that makes all the decisions. It is immensely important to protect oneself from outside forces or evil eyes. Maharishi Yagnavalkya said that this position is appropriate because Shiva's third eye is located here. 
 In fact, our scriptures say that a forehead without a Tilak is as good as a village without a well, a country without a river, a country without justice, wealth not given away as charity, a flower without a scent, etc.
The word Ajna translates to “command” or “perceive.” This reflects the idea that the third eye chakra guides our inner vision, helping us command our thoughts, choices, and insights. 
After applying Tilak, pure thoughts are said to emerge. The application enhances concentration, increases spiritual energy, and wards off negative vibrations, dispelling the effects of evil eyes. 
When you press the Ajneya chakra while applying Tilak, it activates the pineal and pituitary glands. 
Traditionally represented by a lotus with two petals, it symbolizes the balance between intuition (Ida Nadi) and rationality (Pingala Nadi).
 These spots represent the body’s vital energy centers. Applying a Tilak is a small act with massive spiritual rewards. It reminds us that our life is sacred. It keeps us grounded while we aim for the sky.
Tilak is a tool for transformation. It sits right on the Ajna Chakra, or the Third Eye. This point is the seat of intuition and memory. When you apply a Tilak, you activate a chakra point, which brings a natural sense of calm. It helps you focus better and clears mental fog. Most importantly, it improves your focus and aligns your soul with higher consciousness.
The Ajna Chakra is symbolized by a two-petaled lotus flower, each petal representing the Nadis (subtle energy channels) Ida and Pingala. At its center is a sacred symbol, often depicted as a downward-facing triangle or the Om symbol. The chakra's color is typically indigo or deep blue, signifying its connection to wisdom, intuition, and higher consciousness.
Your pineal gland is a tiny endocrine gland in the middle of your brain that helps regulate your body's circadian rhythm by secreting the hormone melatonin. Your pineal gland’s main job is to help control the circadian (every day) cycle of sleep and wakefulness by secreting melatonin
The Pituitary gland, considered the Master gland, secretes hormones that regulate various body functions. It is critical for spiritual awakening and balance.
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